The Influence of Remediation with Bacillus and Paenibacillus Strains and Biochar on the Biological Activity of Petroleum-Hydrocarbon-Contaminated Haplic Chernozem

The effect of bacterial strains on certain genera, both independently and in combination with biochar various options, petroleum hydrocarbon decomposition chernozem the restoration ecological state soil were studied. To simulate contamination, hydrocarbons introduced into amount 5% weight. Strains Bacillus Paenibacillus bacteria (in recommended increased doses × 100) (1% weight) contaminated separately together. It was found that after 30 days, oil content decreased joint introduction an dose Bacillus, Panibacillus by 64%, as well inoculation at 67%. biochar, inoculated BP 100, contributed to increase intensity CO2 emission compared background 5–10%. With + B, stimulation 70%, concentration 100–115%. preparation 100 stimulated activity enzyme 49 61%; preinoculation 27% relative value. most informative biological indicators when introducing ameliorants total number bacteria, length barley roots catalase activity, demonstrating greatest sensitivity. results study should be used for remediation biomonitoring oil-contaminated soils.